Slow growth rate limits the development of seedlings from the Podocarpaceae family. The extended photoperiod (EP) is well known to promote tree seedling growth, but it may also cause the possibility of nutrient dilution without a proper nutrient supply regime. Exponential fertilization (EF) has been proven to counter nutrient dilution within tree seedlings efficiently. However, no study has determined the response of Podocarpus seedlings to the interaction of EF and photoperiod. We fed 13-month old Buddhist pine (Podocarpus macrophyllus [Thunb.] D. Don) seedlings using EF applied at rates of 0 (control), 50 (E50), 100 (E100), and 150 (E150) mg nitrogen (N) seedling(-1) for 5 months, during which half of all seedlings were cultured in the natural photoperiod (NP) with the others exposed to EP. The longer photoperiod resulted in greater seedling height and biomass accumulation of Buddhist pine seedlings. Relative to NP, EP increased N content at the whole-plant level but decreased N concentration in the stem and root, which indicated the symptom of N dilution. However, both phosphorus (P) content and P concentration increased in response to the longer photoperiod. EF rates in the E100 and E150 treatments were evaluated to be the sufficiency' ones for both N and P in EP and NP, respectively. In conclusion, EP could stimulate the growth of Buddhist pine seedlings but caused N dilution within them at the same time, and photoperiod would change the nutritional response of these seedlings to EF.